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Residue-specific binding of mAb41 to E2-16Fc and its mutants. (A) Schematic representation of E2-16Fc and its mutants used in Western blot analysis. (B) E2-16Fc and its mutants were purified from cell culture medium by use of protein G magnetic beads. Eluates were separated by SDS-PAGE and probed with either anti-human IgG1 Fc (anti-Fc) or mAb41.
