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Interaction of CD81-Luc with various mutant forms of E2-16Fc. The binding of CD81-Luc to individual mutants of E2-16Fc is indicated by percentages relative to the binding to E2-16Fc (WT). The inset shows the amounts of E2 protein bound to protein G magnetic beads, which were quantitated after Western blot analysis with anti-Fc, using AlphaView Q software (Alpha Innotech Corp.) to normalize the protein input. The data from three independent experiments are presented.
