FIG 2.

oBST2B localizes to the Golgi apparatus. (A) CPT-Tert cells were transfected with oBST2A-HA or oBST2B-HA and analyzed 18 h after transfection by confocal microscopy using, respectively, antibodies against the HA epitope (to detect oBST2A-HA or oBST2B-HA protein) and the Golgi markers: p115, giantin, TGN46, and appropriate secondary conjugated antibodies. Scale bars in all panels represent 10 μm. (B) Colocalization of oBST2A-HA and oBST2B-HA with Golgi markers was measured in at least 50 cells from two independent experiments with Image-Pro Plus software using Pearson's correlation coefficient. Any values above 0.5 were regarded as representing significant colocalization.