FIG 3.

oBST2B localization is altered by treatment with brefeldin A. (A) CPT-Tert cells were transfected with oBST2B-HA expression plasmids. Eighteen hours after transfection, cells were treated or not treated with 200 ng/ml of brefeldin A for 90 min, fixed, and analyzed by confocal microscopy using antibodies to the giantin Golgi marker and the HA epitope as indicated. Two different staining patterns were observed: (i) dispersed within the cytoplasm and (ii) concentrated in a perinuclear region. Scale bars in all panels represent 10 μm. (B) Graph representing the number (%) of cells in which oBST2B-HA and giantin staining were observed to be concentrated as opposed to dispersed. At least 100 cells from two independent experiments were evaluated randomly.