FIG 4.

oBST2A and oBST2B both redistribute intracellular Env and Gag. CPT-Tert cells were transfected with the full-length JSRV₂₁ expression plasmid with either an empty plasmid or expression plasmids for oBST2A-HA or oBST2B-HA. CPT-Tert cells were also transfected with an expression plasmid for oBST2B-HA in order to assess the influence of this protein on the intracellular distribution of cellular cadherins. Upon immunostaining with the appropriate antibody, the intracellular distributions of Env (A), Gag (B), and cadherins (C) were scored and counted as concentrated or dispersed or at the plasma membrane using confocal microscopy. Scale bars in all panels represent 10 μm. Graphs represent the number (%) of cells in which the intracellular distribution of Env (A), Gag (B), or cadherins (C) displays a concentrated, dispersed, or plasma membrane staining pattern. At least 100 cells in random fields from two independent experiments were counted.