FIG 5.
IFN-λ2 and IFN-λ3 treatment decreases hMPV lung viral titer in infected mice. BALB/c mice were treated i.n. with the indicated concentrations of murine recombinant IFN-λ2 (A and B) or IFN-λ3 (C and D) or with a combination of IFN-λ2 plus IFN-λ3 (E and F) for 24 h prior infection with 1 × 107 PFU of hMPV. Lung tissue was collected at day 4 after infection. (A, C, and E) Expression of the hMPV N gene was determined by qRT-PCR. (B, D, and F) Infectious viral particles were titrated on LLC-MK2 cell monolayers by a methylcellulose plaque assay. Data are representative of at least two experiments with similar results (n = 3 to 6 mice/group). The bar graphs represent means ± standard errors of the means. *, P < 0.05; **, P < 0.01; ***, P < 0.001.