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. 2014 Dec 16;89(1):763–775. doi: 10.1128/JVI.01955-14

FIG 1.

FIG 1

Sequence similarity alignment of ORF119L with the dominant negative form of ILK. (A) Phylogenetic tree analysis of ORF119L orthologues from NCBI zebrafish protein database. These orthologues score highest in levels of identity to ORF119L in the BLAST analysis. The GenBank accession numbers of proteins (in order) are as follows: XP_005157118.1, NP_001186697.1, XP_005161412.1, XP_697378.6, XP_001920876.2, XP_003199303.2, XP_005158347.1, XP_001920092.1, NP_899192.1, XP_689875.3, XP_005167841.1, XP_005167842.1, XP_005167840.1, XP_003200555.2, NP_001018164.1, XP_689244.2, XP_005166638.1, XP_005156096.1, NP_001093460.1, XP_002663935.3, XP_001920231.2, XP_005165903.1, XP_005165902.1, XP_005160666.1, XP_696390.3, NP_001020714.1, NP_991159.1, XP_002666119.1, XP_001923751.2, AAL98843, and NP_956865.1. (B) Analysis of the three-ankyrin-repeat-containing domain of ISKNV ORF119L and ILK proteins from zebrafish (zILK; GenBank accession no. AAH56593), mouse (mILK; GenBank accession no. NP_001155196), and human (hILK; GenBank accession no. CAG28601) with the SMART program (http://smart.embl-heidelberg.de). Compared to zILK, mILK, and hILK, the COOH terminus of ISKNV ORF119L lacks a kinase domain. The numbers indicate the positions of amino acid residues. (C and D) Model structures of the 119L 3ANK (C) and hILK 3ANK (D) domains were generated using the SWISS-MODEL Workspace. (E) Multiple-sequence alignment of the three-ankyrin-repeat-containing domains of ISKNV ORF119L and zebrafish, mice, and humans was performed by using the ClustalW program with default settings. 3ANK, three-ankyrin-repeat domain.