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. 2014 Dec 16;89(1):763–775. doi: 10.1128/JVI.01955-14

FIG 5.

FIG 5

ORF119L overexpression affects angiogenesis in zebrafish embryos. (A and B) The ISV structure was examined in Tg(flk1:GFP) transgenic embryos. ISV development was normal in mock vector-injected embryos (A), whereas disorganized ISV was found after ORF119L overexpression (B) at 3 dpf. (C to J) Whole-mount AP staining showing the vessel development in control (C, D, G, and H) and ORF119L-overexpressing (E, F, I, and J) embryos at 3 dpf. SIV was impaired after ORF119L overexpression. (K and L) ISV structure was normal in 119LΔ3ANK-expressing embryos at 3 dpf. ISV, intersegmental vessel; PCV, posterior cardinal vein; DA, dorsal aorta; PD, pronephric duct; SIV, subintestinal vessel. Scale bars = 500 μm (A, B, C, E, G, I, K, and L) and 200 μm (D, F, H, and J).