FIG 1.

T_H plays a role but is not critical in generating anti-VACV CD8_E and/or the maintenance of anti-VACV CD8_M. B6 and MHC-II^0/0 mice were primed i.p. with 5 × 10⁶ PFU VACV strain WR. (A) CD8 T cell responses in spleens were determined at 7 dpp. Representative flow cytometry plots on the left show IFN-γ and GzB expression after in vitro restimulation with VACV-infected DC2.4 cells (upper row) and staining with K^b-TSYKFESV dimers (bottom row) by gated CD8⁺ cells. The summary graphs on the right show the frequency (top row) and number in millions (bottom row) of IFN-γ⁺, GzB⁺, and K^b-TSYKFESV⁺ CD8⁺ T cells for three mice/group. (B) Frequency of K^b-TSYKFESV⁺ CD8_M at different dpp. NT, not tested because the data are from spleens pooled from 2 or 3 mice. (C) Frequency of K^b-TSYKFESV⁺ CD8_M expressing the indicated cell surface molecules at 60 dpp. Data are representative of results from two or three similar experiments. n.s., not significant.