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Surface expression levels of ICAM-1. The stable ICAM-1-expressing 293T cell line (A) and PBMCs from three healthy donors (B) were incubated with either an R-phycoerythrin-conjugated mouse anti-human ICAM-1 antibody (black lines) or an isotype-matched irrelevant antibody (used as a control) (gray areas). The results shown are representative of data from 1 out of a total of 3 independent experiments.
