FIG 3.
Immunocapture of VLPs carrying mutations in the MAΔ44-132 region. (A) VLPs produced by 293T cells stably expressing ICAM-1 were captured by using magnetic beads coated with either an anti-ICAM-1 (R6.5) or isotype-matched irrelevant biotinylated antibody. Next, VLPs were estimated by Western blotting using an anti-p24 monoclonal antibody (183-H12-5C) recognizing both the Pr55Gag precursor polyprotein and p24. The primary antibody was revealed with a horseradish peroxidase-conjugated anti-mouse secondary antibody. (B) The Pr55Gag precursor polyprotein signals were determined by densitometry analyses of Western blot films using ImageJ software. Data shown represent results from three independent experiments for each mutant, and error bars indicate standard deviations.