FIG 7.

Immunocapture of NL4.3-derived WT and mutant viruses into which MA acidic residues were replaced by alanines. NL4.3-derived WT virions and mutant virus particles carrying the listed alanine substitutions were prepared in 293T cells stably expressing wild-type ICAM-1. Next, virus stocks were captured by using magnetic beads coated with an anti-ICAM-1 antibody (R6.5) or an isotype-matched irrelevant biotinylated antibody (used as a control). Precipitated viral entities were quantified with a homemade p24 ELISA. Data shown represent the means ± standard deviations of data from triplicate samples and are representative of three independent experiments. Statistical analyses were made by using one-way ANOVA, and asterisks denote a statistically significant difference between the NL4.3 WT and the listed mutants (***, P < 0.005; ****, P < 0.0001).