FIG 9.

Immunocapture of NL4.3 WT virus produced in 293T cells transiently expressing wild-type or mutated ICAM-1. (A) NL4.3 WT virus particles were produced in 293T cells transiently expressing either WT or mutated ICAM-1. Next, virus stocks were captured by using magnetic beads coated with an anti-ICAM-1 antibody (R6.5) or an isotype-matched irrelevant biotinylated antibody (used as a control). Precipitated viral entities were quantified with an in-house p24 ELISA. Data shown represent the means ± standard deviations of data from triplicate samples and are representative of three independent experiments. (B and C) Flow cytometry analyses of surface expression of ICAM-1 in 293T cells transiently expressing WT (B) or mutated (C) ICAM-1. WT and mutated ICAM-1 are shown as solid black lines, while negative controls are presented as gray-filled histograms. Data shown represent cell surface expression of ICAM-1 at 2 days following transfection. Statistical analyses were made by using the Student t test, and asterisks denote statistically significant difference between the NL4.3 WT and the listed mutant (****, P < 0.0001).