TABLE 1.
Primers used for mutagenesis
| Mutant | Primer sequence |
|
|---|---|---|
| Forwarda | Reverse | |
| NL4.3Δ44-132 | CCTATAGTGCAGAACCTCCAG | TCGTTCTAGCTCCCTGCTTGC |
| NL4.3 Del 102-114 | GCACAGCAAGCAGCAGCT | TAAGGCTTCCTTGGTGTCTTT |
| D102A/E105A/E106A | AAGGAAGCCTTAGCTAAGATAGCAGCAGAGCAAAACAAAAGT | GGTGTCTTTTACATCTATCCTTTGATGCACACAATAGAG |
| D102A/E105A/E106A/E107A | AAGGAAGCCTTAGCTAAGATAGCAGCAGCACAAAACAAAAGT | GGTGTCTTTTACATCTATCCTTTGATGCACACAATAGAG |
| D102A/E105A/E106A/E107A/S111A | GCAGCACAAAACAAAGCTAAGAAAAAGGCACAGCAAGCA | TGCTATCTTAGCTAAGGCTTCCTTGGTGTCTTTTACATC |
| CD54 Del 505-514 | CAACAGGCCCAAAAAGGG | GTTATAGAGGTACGTGCTGAGGCC |
| CD54 R505,507,513A K508,510,511A | TACCTCTATAACGCCCAGGCCGCAATCGCCGCATACGCACTACAACAGGCC | CGTGCTGAGGCCTGCAGTGCCCATTATGACTGCGGCTGCTAC |
a
Underlined nucleotides correspond to the mutated codons for alanine substitution.