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. 2014 Dec 16;89(1):751–762. doi: 10.1128/JVI.02430-14

FIG 5.

FIG 5

Auto-feedback loop of IFN-I during acute SIV infection of AGMs. (A) IFN-I levels present in the plasma of eight acutely SIV-infected AGMs. (B) Correlation between IFN-I levels in the plasma of AGMs during acute SIVagm infection in vivo and the amount of IFN-I produced upon in vitro HSV stimulation. (C) Plasma collected from healthy AGMs, acutely infected AGMs, and chronically infected AGMs was diluted to 5% in RPMI and added to PBMCs of eight healthy AGMs. After 4 h of incubation, cells were stimulated with HSV-1, and after another 18 h, supernatants were collected for IFN-I quantification. PBMCs were incubated with anti-IFN-αR2 or with an irrelevant antibody as a negative control at 10 μg/ml for 1 h to block IFN-α signaling before adding plasma. Symbols represent individual animals. (D) Correlation between mRNA levels of known HSV sensors in PBMCs of AGMs during acute SIVagm infection in vivo and the amount of IFN-I produced upon in vitro HSV stimulation of the same PBMCs. **, Wilcoxon, P < 0.01.