FIG 1.

Detection of GF-1 cell membrane proteins (GFMPs) with interactivity with NNV capsid protein by VOPBA assay. Total GFMPs were resolved by 10% SDS-PAGE and transferred to PVDF membrane. Purified-NNV overlay was then performed. Bound virions were detected by rabbit anti-NNV polyclonal antibodies. Lanes: M, molecular mass marker; 1, Western blot of purified NNV using rabbit anti-NNV polyclonal antibodies; 2, VOPBA without purified NNV overlay; 3, VOPBA with NNV overlay; 4, Coomassie blue-stained profile of an electrophoresis gel similar to that used to excise the corresponding bands for LC-MS/MS analysis.