FIG 3.

siRNA downregulation of GHSC70 and GVDAC2 gene expression and its effects on NNV replication. GF-1 cells were separately transfected with GHSC70- and GVDAC2-specific siRNAs for downregulation, and nonsilencing siRNA (NS-siRNA) was used as a negative control. (A, B) The gene expression levels of GHSC70 or GVDAC2 (A) and their protein levels (B) were separately analyzed by real-time PCR and Western blotting at 24 h posttransfection. (C, D) After siRNA transfection, the cells were infected with NNV (MOI of 100), and NNV RNA2 expression levels (C) and viral titers (D) were examined at 24 h postinfection. The level in the negative control was regarded as 1. Error bars indicate standard deviations. *, P < 0.05; **, P < 0.01 (n = 3).