FIG 7.

Localization of GHSC70 and NNV analyzed by immunofluorescence staining. GF-1 cells were incubated with NNV (MOI of 1,000) on ice for 1 h (bottom). Cells without NNV incubation (top) were used as a negative control. Cells were fixed with formalin without Triton X-100 permeabilization and immunostained with rabbit anti-GHSC70 and mouse anti-NNV antibodies following rhodamine-labeled and FITC-conjugated antibody staining, respectively. Cell nuclei were stained with Hoechst 33258. Samples were observed under a confocal microscope. Bar = 10 μm.