FIG 5.

Identification of Shibuyunji virus (SHBV) from a field tick sample in Zambia. (A) The Shibuyunji district in Zambia is indicated by the black star. The Zambia location map by NordNordWest was obtained from Wikimedia Commons and modified by the authors. (B) RT-PCR was performed with field tick samples corresponding to homogenized single tick samples (CZCZT13-1 to CZCZT13-25). (C) Four out of eight RT-PCR fragments were sequenced (no sequencing reads was obtained with the other four fragments), and a phylogenetic tree was constructed based on these sequences, as well as reference sequences, using the maximum likelihood (ML) method with 1,000 bootstraps. Bootstrap probabilities above 70% are indicated near the branches. SFTSV, severe fever with thrombocytopenia syndrome virus.