. 2014 Dec 16;89(1):594–604. doi: 10.1128/JVI.02704-14

TABLE 1.

Primers used in the RT-PCR

Primer set	Primer	Binding region^a	Sequence^b (5′ → 3′)
ppL1	TBPVL2759F	2786–2803	CAGCATGGIGGICTIAGAGAGAT
	TBPVL3267R	3281–3300	TGIAGIATSCCYTGCATCAT
ppL2	HRT-GL2759F	2786–2814	CAGCATGGIGGIYTIAGRGAAATYTATGT
	HRT-GL3276R	3281–3309	GAWGTRWARTGCAGGATICCYTGCATCAT

Nucleotide positions in the L segment RNA (cRNA) of Uukuniemi virus strain S23 (GenBank accession number D10759).

Nonstandard nucleotides are as follows; I, inosine; R, adenine (A) and guanine (G); S, G and cytosine (C); W, A and thymine (T); Y, T and C.