TABLE 1.
Replication of reassortant viruses in micea
| Virus | Mean titer (log EID50/ml) ± SD |
No. of mice with infection in organ/total no. of mice (titer[s] in individual positive mice) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Lung |
Heart |
Liver |
Spleen |
Kidney |
||||||
| 3 dpi | 5 dpi | 3 dpi | 5 dpi | 3 dpi | 5 dpi | 3 dpi | 5 dpi | 3 dpi | 5 dpi | |
| rDK1 | 5.7 ± 0.4 | 6.2 ± 0.4 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| rDK1-MA | 8.5 ± 0.3 | 8.0 ± 0.5 | 2/3 (1.3, 2.3) | 2/3 (2.3, 3.5) | 0/3 | 1/3 (2.8) | 1/3 (2.0) | 1/3 (1.5) | 0/3 | 1/3 (1.8) |
| DK1/MAPB2 | 8.1 ± 0.4b | 7.6 ± 0.3b | 1/3 (2.5) | 2/3 (2.3, 2.9) | 0/3 | 0/3 | 1/3 (1.5) | 1/3 (1.5) | 0/3 | 0/3 |
| DK1/MAPA | 6.5 ± 0.4c | 6.3 ± 0.7 | 1/3 (2.5) | 1/3 (2.5) | 0/3 | 0/3 | 1/3 (1.5) | 0/3 | 0/3 | 0/3 |
| DK1/MAHA | 6.3 ± 0.4 | 6.3 ± 0.3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| DK1/MANP | 5.5 ± 0.7 | 5.4 ± 0.4 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| DK1/MANA | 5.2 ± 0.3 | 5.4 ± 0.4 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| DK1/MAM | 5.3 ± 0.4 | 5.7 ± 0.5 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| MA/DK1 PB2 | 7.3 ± 0.3b | 7.3 ± 0.5 | 0/3 | 1/3 (2.3) | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| MA/DK1 PA | 8.1 ± 0.6 | 7.5 ± 0.4 | 1/3 (2.0) | 1/3 (0.8) | 1/3 (1.3) | 0/3 | 2/3 (1.3, 1.9) | 1/3 (0.8) | 0/3 | 1/3 (1.3) |
| MA/DK1 HA | 8.2 ± 0.5 | 7.4 ± 0.3 | 2/3 (2.5, 3.3) | 2/3 (1.3, 3.3) | 0/3 | 1/3 (1.3) | 1/3 (1.3) | 1/3 (0.8) | 0/3 | 1/3 (1.5) |
| MA/DK1 NP | 8.3 ± 0.5 | 8.3 ± 0.4 | 2/3 (1.2, 1.8) | 3/3 (3.8 ± 0.3)d | 0/3 | 1/3 (2.0) | 1/3 (1.3) | 1/3 (1.8) | 0/3 | 1/3 (2.0) |
| MA/DK1 NA | 7.5 ± 0.5 | 8.3 ± 0.5 | 1/3 (0.8) | 3/3 (3.1 ± 1.0) | 1/3 (2.3) | 2/3 (2.3, 4.3) | 1/3 (1.8) | 1/3 (1.3) | 0/3 | 2/3 (1.3, 3.3) |
| MA/DK1 M | 8.1 ± 0.5 | 8.2 ± 0.8 | 2/3 (3.0, 3.4) | 3/3 (3.7 ± 0.4) | 1/3 (2.5) | 0/3 | 1/3 (0.8) | 0/3 | 0/3 | 1/3 (1.8) |
| DK1/MA PB2+PA | 8.3 ± 0.3b | 8.0 ± 0.5b | 1/3 (1.8) | 2/3 (1.3, 3.5) | 0/3 | 0/3 | 0/3 | 1/3 (2.3) | 0/3 | 0/3 |
| DK1/MA PB2+HA | 8.4 ± 0.6b | 7.5 ± 0.9c | 1/3 (2.0) | 2/3 (2.3, 3.3) | 0/3 | 0/3 | 0/3 | 1/3 (1.8) | 1/3 (2.3) | 0/3 |
| DK1/MA PA+HA | 7.8 ± 0.8b | 7.5 ± 0.5c | 0/3 | 1/3 (2.3) | 0/3 | 0/3 | 1/3 (2.0) | 0/3 | 0/3 | 0/3 |
| DK1/MA PB2+PA+HA | 8.5 ± 0.3b | 7.8 ± 0.3b | 0/3 | 3/3 (2.3 ± 0.4) | 0/3 | 1/3 (1.3) | 0/3 | 1/3 (1. 5) | 1/3 (1.8) | 0/3 |
| MA/DK1 PB2+PA | 6.0 ± 0.3b | 7.3 ± 0.7 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| MA/DK1 PB2+HA | 6.5 ± 0.4b | 7.3 ± 0.9 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| MA/DK1 PA+HA | 7.5 ± 0.7c | 7.0 ± 0.9 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
| MA/DK1 PB2+PA+HA | 6.3 ± 0.7b | 7.3 ± 0.3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
Groups of mice were infected with 105 EID50 of each virus. Three mice from each group were euthanized, and the lungs, heart, liver, spleen, and kidneys were collected and homogenized in 1 ml of phosphate-buffered saline for virus titration in eggs at 3 and 5 days p.i. The lower limit of detection was 100.8 EID50/ml for these samples. dpi, days postinoculation.
Significantly different (P < 0.01) from titers in lungs of mice that received the corresponding parental virus.
Significantly different (P < 0.05) from titers in lungs of mice that received the corresponding parental virus.
When all three animals in a treatment group had positive virus titration results for an organ, the mean ± standard deviation is reported in parentheses (rather than results for individual animals).