Figure 5.

Mutations predicted in LTEE 500- and 1000-generation populations. (A) Total numbers of mutations detected across all 12 populations at each time point in each category. Single nucleotide (SN) mutations are single-base substitutions, insertions, and deletions. Structural variation (SV) mutations are insertions and deletions of ≥2 bases and other events such as IS element insertions, all of which are predicted from new sequence junctions by breseq. (B) Total frequencies of mutations detected across all 12 populations at each time point in each category. (C) Breakdown of the spectrum of molecular effects of all mutations detected within the 12 LTEE populations. (D) Fraction of total genetic variation contributed by SN vs. SV mutations in each LTEE population sample, weighted by mutation frequencies.