Table I.

Novel CD4 T cell epitopes.

ORF	Amino Acids	Sequence	Function	Gene Expressiona	Stimulated by AC-RTA (12 d p.i.)
M2	124–138	NSEPVYIQPISTRSL	Immune regulation/latency	E-L	No
M4	156–170	RSSQWEVAFSVVSKT	Immune regulation	IE	Yes
ORF6	593–607	CNTNWLPPCPIIHNL	ssDNA binding protein	E-Lb	Yes
ORF8	285–299	GQSRTFLETPSATYS	glycoprotein B	L	No
ORF8	326–340	HEHSYHFVANEVTAT	glycoprotein B	L	No
ORF8	482–496	TLMWYELSKVNPTSV	glycoprotein B	L	Yes
ORF17	310–324	ITNHAAFSTPGAGLS	Serine protease/capsid morphogenesis	L	No
ORF21	582–596	LLHKFCYELSKPHMV	Thymidine kinase	E-L	Yes
ORF25	1215–1229	KLLYDHGQPDPAYEF	Major capsid protein	E-L	Yes
ORF45	183–197	ESSRILKTPAPISGN	Tegument protein	L	Yes
ORF48	255–269	DTSSTWTWPAARIAE	Tegument protein	L	Yes
M7	111–125	PTEADPKAAPSAGHV	glycoprotein 150	L	No
ORF52	51–65	SIIVSSSRALGAVAM	Tegument protein	L	Yes
ORF61	343–357	EQGAYEAVVPIKSVM	Ribonucleotide reductase large subunit	L	Yes
ORF61	691–705	LFLNEDYASSASNIK	Ribonucleotide reductase large subunit	L	Yes
ORF68	78–92	LCFYVLHAPVTWSAT	DNA packaging protein	E	No
ORF75b	1020–1034	MLQYAGFLPEIVHSS	Tegument protein	E	Yes

^aIE, immediate-early; E, early; E-L, early-late; L, late (16)

^bORF6 gene expression has been characterized as E (41), E-L (42), L (16) and kinetic cluster II (mostly corresponding to E and E-L genes) (43)