Fig. 1.

Flow cytometric analysis of immature and mature dendritic cells. The dendritic cells were cultured as described in “Materials and Methods.” Immature and mature dendritic cells were harvested, stained with fluorescein isothiocyanate–labeled anti-CD11c (dendritic cell marker) monoclonal antibody and phycoerythrin-labeled anti-CD83 (maturation marker of dendritic cells) monoclonal antibody, and then analyzed by flow cytometry. Expression rates of CD11c and CD83 in unpulsed dendritic cells (i.e., immature dendritic cells) and RM-1 tumor lysate–pulsed dendritic cells (i.e., mature dendritic cells) were, respectively, 83.2% (i.e., 3.4% + 79.8%) and 3.4% for CD11c, and 84.1% (i.e., 66.7% + 17.4%) and 66.7% for CD83, respectively.