Figure 4. Putative role of Wnt signalling networks in paracrine regulation of titrated adipogenesis.
(A) Paracrine regulation of adipogenesis by Wnt10b. Co-culture experiments demonstrate that Wnt10b-expressing cells do not differentiate, but can also inhibit the adipogenic potential of neighbouring preadipocytes. Co-cultures of preadipocytes expressing either empty vector (EV), Wnt10b or GFP (green fluorescent protein) only were induced to differentiate into adipocytes. Similar results were observed when Wnt10b-expressing preadipocytes were replaced by preadipocytes in which Dact1 was knocked down [98]. Images were generated using Cell-IQ from Chipman Technologies. (B) The paracrine Wnt signalling network mediates cross-talk between preadipocytes and maturing adipocytes. This network is also nutritionally regulated and may regulate titrated adipocyte recruitment in vivo [98].
