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Steady-state constants for catalysis of the hydration of CO2 by variants of HCA II obtained by stopped-flow spectrophotometry at 25 °C and pH 8.3.
| Variant | kcat/Km (μM−1s−1) |
kcat μs−1) |
|---|---|---|
| Wild-typea | 120 | 1.0 |
| V143A | 42 ± 1b | 0.5 ± 0.2b |
| V143I | 9.3 ± 0.3 | 0.7 ± 0.1 |
| V143L | 5.5 ± 0.1 | 1.1 ± 0.3 |
From Steiner et al. (31)
Standard errors were determined from fits of steady-state data.
