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. 2014 Nov 27;15(1):1030. doi: 10.1186/1471-2164-15-1030

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© Nwafor et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantitative real-time PCR validation of RNA-Seq data. Relative expression profile of 14 genes shows the expression fold change (FC) in the pairwise comparison between developmental stages for the wild-type and the mutant. Histograms represent expression fold changes as assessed by real-time PCR (by using REST), data are reported as means ± SE of three technical replicates (left axis). Green lines represent expression fold changes as assessed by RNA-Seq (by using DESeq, right axis). Blue column with error bar corresponds to the first biological replicate, while red column corresponds to the second biological replicate on which RNA sequencing was carried out.