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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Neurol. 2014 Jul 25;261(10):1929–1938. doi: 10.1007/s00415-014-7429-1

Figure 5. Representative current traces (a, c) and Gj-Vj relations (b, d), for Arg98Leu expressed in Neuro2a cells and paired homotypically or heterotypically with Cx43 WT.

Figure 5

a. Macroscopic junctional currents for homotypically paired cells expressing the Cx47Arg98Leu mutant are similar to those seen for Cx47WT. b. Steady state Gj-Vj relations for homotypically paired cells expressing the Arg98Leu mutant are similar to those seen for Cx47WT, though the Arg98Leu mutant shows a slightly decreased Gmin. c. Macroscopic junctional currents between heterotypic cells pairs in the Cx43WT/Cx47Arg98Leu configuration are similar to those seen for Cx43WT/Cx47WT. d. Steady state Gj-Vj relations for heterotypic Cx43WT/Cx47Arg98Leu junctions are, for the most part, similar to those seen for Cx43WT/Cx47WT junctions though the negative limb of the Cx43WT/ Arg98Leu Gj-Vj relation (corresponding to the closure of the Cx47 hemichannel) shows a modest inward shift (indicating closure at smaller Vj) and a slightly decreased Gmin compared to that for Cx43WT/Cx47WT. Filled squares are average normalized conductances. Error bars represent SEM. Solid lines are Boltzmann fits to the steady state Cx47Arg98Leu/Cx47Arg98Leu and Cx43WT/Cx47Arg98Leu data. Dashed lines are Boltzmann fits to steady state Cx47WT/Cx47WT and Cx43WT/Cx47WT Gj-Vj relations.