1.
|
αA- cry
|
NM_012534.2
|
Sense: TGGCAAACACAACGAGAGGCA
Antisense: GCCAGCATCCAAGCCAGACT
|
58 °C/35
|
2.
|
βB1 -cry
|
NM_012936.2
|
Sense: CACCAACACAGCATCAGAAACCAT
Antisense: GTTTTCCTGCTCAAAGACGACCA
|
60 °C/35
|
3.
|
γD- cry
|
NM_033095.1
|
Sense primer: CGACTCTGTCCGCTCCTGCC
Antisense: TACTCCCCGGGCCTCAGCAG
|
59 °C/35
|
4.
|
Cat
|
NM_012520.1
|
Sense: ACTGGGACCTCGTGGGAAAC
Antisense: TCTGGAATCCCTCGGTCGCT
|
57 °C/40
|
5.
|
SOD-1
|
NM_017050.1
|
Sense: CGGTGCAGGGCGTCATTCACTT
Antisense: CTCTTCATCCGCTGGACCGCC
|
58 °C/35
|
6.
|
Gpx
|
NM_030826.3
|
Sense: CACCACGACCCGGGACTACA
Antisense: AGGTAAAGAGCGGGTGAGCC
|
58 °C/35
|
7.
|
Fas
|
NM_139194.2
|
Sense: TTCGAATGCAAGGGACTGAT
Antisense: TGGGCCCACTTGATATAACC
|
56 °C/35
|
8.
|
TNF-α
|
NM_012675.2
|
Sense: TAGCAAACCACCAAGCAGAG
Antisense: CCTTGAAGAGAACCTGGGAG
|
60 °C/40
|
9.
|
Caspase-8
|
NM_022277.1
|
Sense: TGGGAAGGATCGACGATTAC
Antisense: CCACATGTCCTGCATTTTGA
|
58 °C/35
|
10.
|
Caspase-3
|
NM_012922.2
|
Sense: GGTTCATCCAGTCACTTTGC
Antisense: CCAGGGAGAAGGACTCAAAT
|
60 °C/40
|
11.
|
Bid
|
NM_022684.1
|
Sense: CTAGGGACATGGAGAACGAC
Antisense: CCCTCACGTAGGAGAATAGG
|
60 °C/40
|
12.
|
Bad
|
NM_022698.1
|
Sense: TCCGAAGAATGAGCGATGAA
Antisense: TTCCCCAAATTTCGATCCCA
|
59 °C/40
|
13.
|
Bax
|
NM_017059.2
|
Sense: CGATGAACTGGACAACAACA
Antisense: AGCAAAGTAGAAAAGGGCAA
|
60 °C/40
|
14.
|
Bcl-2
|
NM_016993.1
|
Sense: GGGATGCCTTTGTGGAACTA
Antisense: CTCACTTGTGGCCCAGGTAT
|
60 °C/35
|
15.
|
Caspase-9
|
NM_031632.1
|
Sense: GAACGACCTGACTGCTAAGA
Antisense: GAACGACCTGACTGCTAAGA
|
57 °C/35
|
16.
|
Cytochrome- C
|
JF919282.1
|
Sense: TTGTTCAAAAGTGTGCCCAG
Antisense: GTAAGAGAATCCAGCAGCCT
|
61 °C/40
|
17.
|
NFkB
|
NM_001276711.1
|
Sense: ACACCTCTACACATAGCAGC
Antisense: GGCACTACTCCCTCATCTTC
|
60 °C /40
|
| 18. |
β-actin |
NM_001109553.1 |
Forward primer: AGTCCCTCACCCTCCCAAAA
Reverse primer: AGCAATGCTGTCACCTTCCC |
60 °C /40 |
