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CDC42 silencing decreases N-myc expression. (A) Immunoblotting for protein levels of Twist, AKT2 and N-myc in BE(2)-C cells demonstrated a decrease in all three of these signaling mediators with CDC42 inhibition. (B) BE(2)-M17 cells exhibited decreased N-myc expression after shCDC42, and no changes in Twist or AKT2. (C) Neither Twist, AKT2 nor N-myc levels were affected by CDC42 silencing in SK-N-AS cell line. In all cases, β-actin levels were used as a loading control.
