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. Author manuscript; available in PMC: 2015 Dec 28.
Published in final edited form as: Cancer Lett. 2014 Sep 28;355(2):210–216. doi: 10.1016/j.canlet.2014.08.033

Fig. 5.

Fig. 5

Twist, AKT2 or N-myc overexpression in CDC42-silenced cells does not restore neuroblastoma growth. (A) Immunoblotting confirmed transient Twist overexpression (pCMV6-XL5-Twist) in shCDC42. β-actin indicated relatively equal protein loading. Twist overexpression did not induce increases in shCON cell growth. CDC42 inhibition decreased cell growth; Twist overexpression in the shCDC42 cells did not restore the decreased cell growth. (B) Immunoblotting confirmed transient AKT2 overexpression (pcDNA3- AKT2) in shCDC42. AKT2 overexpression increased cell growth in shCON cells but not in shCDC42 cells. AKT2 overexpression in the shCDC42 cells did not restore cell growth (*p < 0.05 vs. AKT2 control vector). (C) Immunoblotting confirmed transient N-myc overexpression (pCMV6-XL4-N-myc) in shCDC42. N-myc overexpression increased cell growth in shCON cells but not in shCDC42 cells. N-myc overexpression in the shCDC42 cells did not restore cell growth (*p < 0.05 vs. N-myc control vector).