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Time-resolved absorption spectra of E181Q at 233 K following illumination with 495 nm light (A). The difference spectra presented in panel (B) was obtained by subtracting the dark state spectrum from the spectra of each time point. While the blue-shifted species is accounted for by the evolution of Lumi (495 nm) over 85 min, the origin of the red-shifted species with a difference spectrum maximum at 575 nm is currently unknown.
