Table 2.
Essential genes identified on different mediums which are high homology with DEG
| Gene | Enzyme | Essentiality | Subsystem | |
|---|---|---|---|---|
| MG | YE | |||
| MA-133-4 | Ribose-5-phosphate isomerase | E | E | Carbohydrate metabolism |
| MA-090-485 | UTP-glucose-1-phosphate uridylyltransferase | E | E | |
| MA-101-200 | Mannose-6-phosphate isomerase | E | E | |
| MA-120-141 | Phosphoacetylglucosamine mutase | E | E | |
| MA-184-380 | Acetyl-CoA carboxylase | E | E | |
| MA-072-196 | Argininosuccinate lyase | E | NE | Amino acid metabolism |
| MA-213-50 | Adenylosuccinate synthase | E | E | |
| MA-213-539 | Adenylosuccinate lyase | E | E | |
| MA-326-6 | Aspartate carbamoyltransferase | E | E | |
| MA-334-434 | Phosphoribosylanthranilate isomerase | E | NE | |
| MA-120-148 | 2-Acetolactate methylmutase | E | NE | |
| MA-090-434 | Dihydroxy-acid dehydratase | E | NE | |
| MA-139-331 | Homoaconitate hydratase | E | NE | |
| MA-323-77 | Saccharopine dehydrogenase | E | NE | |
| MA-326-106 | Ornithine carbamoyltransferase | E | NE | |
| MA-101-393 | Imidazole-4-carboxamide isomerase | E | NE | |
| MA-184-558 | Imidazoleglycerol-phosphate dehydratase | E | NE | |
| MA-090-452 | Glutamine amidotransferase:cyclase | E | NE | |
| MA-320-159 | 3-dehydroquinate synthase | E | NE | |
| MA-139-157 | Chorismate synthase | E | NE | |
| MA-073-62 | 3-deoxy-7-phosphoheptulonate synthase | E | NE | |
| MA-213-547 | Tryptophan synthase | E | NE | |
| MA-297-40 | Chorismate mutase | E | NE | |
| MA-120-157 | Thioredoxin reductase | E | E | |
| MA-173-30 | Purine-nucleoside phosphorylase | E | E | Nucleotide metabolism |
| MA-213-65 | IMP dehydrogenase | E | E | |
| MA-120-261 | Nucleoside diphosphate kinase | E | E | |
| MA-139-347 | Guanylate kinase | E | E | |
| MA-120-138 | Ribonucleoside-diphosphate reductase | E | E | |
| MA-055-211 | Ribonucleoside-diphosphate reductase | E | E | |
| MA-323-58 | Ribonucleoside-diphosphate reductase | E | E | |
| MA-334-356 | Thymidylate synthase | E | E | |
| MA-153-455 | 3(2),5-bisphosphate nucleotidase | E | NE | |
| MA-111-23 | phosphoadenylyl-sulfate reductase | E | NE | Energy metabolism |
| MA-182-360 | 6,7-dimethyl-8-ribityllumazine synthase | E | E | Cofactors and vitamins |
| MA-184-368 | Riboflavin synthase | E | E | |
| MA-210-311 | Aspartate dehydrogenase | E | E | |
| MA-055-340 | Fatty-acyl-CoA synthase | E | E | Lipid metabolism |
| MA-162-131 | Palmitoyl-protein thioesterase | E | E | |
| MA-334-239 | Very-long-chain enoyl-CoA reductase | E | E | |
E: essential gene; NE: non-essential gene. MG: minimal medium; YE: yeast extract medium.