Figure 3.

Regulation of natalizumab-associated human miRNA targets in a preclinical model of MS. Naïve SJL/J mice received activated encephalitogenic T lymphocytes (stimulated with PLP_139–151) to induce adoptive transfer experimental autoimmune encephalomyelitis (EAE) or PBS only (controls). RNA from splenocytes was isolated at different time points during disease, and data show mean fold changes in expression normalized to that of the housekeeping genes +SEM for duplicate ΔCT values per mouse (n = 3 for control group; n = 5 for day 13 and day 50 groups; asterisks denominate statistical significance, P < 0.05). MS, multiple sclerosis; PLP, proteolipid protein.