Figure 5.

BRET competition analysis. The basal BRET signal obtained after the transient co-expression of LINGO-1-RLuc and LINGO-1-YFP in HEK-293 cells (yielding to around 50% of the BRETmax) is significantly reduced by the transfection of increasing concentrations (0 to 2600 ng) of both the full length of LINGO-1 (A) and its truncated form (LINGO-1-ΔCter) (B). The specificity of these inhibitions has been demonstrated by the lack of effect of trkB on the basal BRET signal (C). Values were expressed as the percentage of BRET signal measured in the absence of protein competitors (control) from three independent experiments read in triplicate. The expressions of the transfected HA-tagged competitors were confirmed by Western blot analysis (upper panels). The average of basal BRET signal value from three experiments was 915 ± 31 mBU. ^*P < 0.05; ^**P < 0.01, ^***P < 0.001, compared with no competitor.