Disruption of Gln3p and Gat1p transcription factors affect LD dynamics. gln3Δ, gat1Δ, and gln3 Δgat1Δ strains and the isogenic strains were grown in YPD, and the LD dynamics and growth were monitored. (A) gln3Δ (○) and gat1Δ (△) strains and the isogenic WT (BY4741) strain (gray). (B) The gln3Δ gat1Δ strain (black) and its isogenic WT (TB50-a) strain (gray). Data are from three independent experiments ± standard deviations (*, P < 0.05; **, P < 0.01; ***, P < 0.001).