FIG 4.

Increased distance between URS1 and URS2 reduces HO expression and SBF binding. (A) The diagram of the HO promoter has rectangles indicating URS1 and URS2, lines indicating Swi5 site locations in URS1, numbers indicating SBF sites in URS2, and a rectangle between URS1 and URS2 indicating the insertions. HO mRNA levels were measured for strains with HO promoters with insertions of the indicated size. (B) The diagram is as described for panel A, with the location of ChIP primers indicated. Binding of the Swi4-V5 subunit of SBF to the indicated versions of the HO promoter was determined by ChIP followed by qPCR. Location of ChIP primers is indicated on the diagram.