TABLE 2.
Plasmid list
| Plasmid | Description |
|---|---|
| M1679 | HO (nt −1902 to −929), in BSKS+ |
| M2775 | CDC39, in pRS316 |
| M2817 | HO (nt −3553 to +2584), in pUC18 |
| M5488 | HO (nt −1930 to −155), with MfeI/BsrG1/MluI/NheI/NotI polylinker at −1052, in pRS306 |
| M5536 | HO (nt −1948 to +2584), with −929 to −233 deleted and replaced by −530 to −229, −927 to −532), in pBR322 |
| M5543 | HO (nt −1930 to −155), with 900 bp from CDC39 inserted at −1052, in pRS306 |
| M5544 | HO (nt −1930 to −155), with 700 bp from CDC39 inserted at −1052, in pRS306 |
| M5545 | HO (nt −1930 to −155), with 500 bp from CDC39 inserted at −1052, in pRS306 |
| M5546 | HO (nt −1930 to −155), with 300 bp from CDC39 inserted at −1052, in pRS306 |
| M5588 | HO (nt −1930 to −155), with 221 bp from CDC39 inserted at −1052, in pRS306 |
| M5543 | HO (nt −1930 to −155), with 900 bp from CDC39 inserted at −1052, in pRS306 |
| M5715 | HO (nt −592 to −126), with four mutant Sbf sites, in pUC57-AMP |
| M5717 | HO (nt −3553 to +2584), with first four Sbf sites mutated, in pUC18 |
| M5718 | HO (nt −3553 to +2584), with first five Sbf sites mutated, in pUC18 |
| pCH341 | HO (nt −1930 to −155), with NotI site at −1052, in pRS306 (provided by Pam Geyer) |
| pCORE-UH | Kluvermyces lactis URA3 and HphMX4, in pFA6 (see reference 27) |
| pZC09 | C-terminal V5 tagging vector with TEV site, Gly linker, and His3MX marker (provided by Zaily Connell and Tim Formosa) |