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. 2015 Jan 21;11(1):e1004602. doi: 10.1371/journal.ppat.1004602

Figure 5. AtXB4 and PLL4/5 play a role in EFR-mediated signaling.

Figure 5

A,D. Elf18-induced ROS burst. Leaf discs were treated with 100nM elf18 treatment. Values are averages ±SE (n = 3, representing independent biological experiments with 12 samples in each experiment). Plant lines were not statistically different, p > 0.05 (two-way ANOVA). The reduced ROS burst in Atxb24-2 and pll4-1 pll5-1 lines was observed in 5/6 and 6/7 experiments, respectively. B,E. Seedling growth inhibition in response to increasing dose of elf18. Seedlings were grown in the presence of elf18 and seedling fresh weight recorded 10 days post-treatment. Values are averages ±SE (n = 12). Plant lines were not statistically different, p > 0.05 (two-way ANOVA). The experiment was repeated three times with similar results. C,F. Elf18-induced resistance to Pto DC3000. Plants were pre-treated with mock or 1 μM elf18 for 24 h before infection with Pto DC3000 (OD600 = 0.0002). Bacterial populations were scored at 2 dpi. Values are averages ±SE (n = 4). Letters indicate statistically significant differences from Tukey’s HSD mean separation (p < 0.05). Infections were repeated at least four times with similar results.