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. 2014 Dec 15;14:488. doi: 10.1186/1472-6882-14-488

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© Li et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vivo KSY treatment reduces Ki-67 expression and increases the expression of p53 and p21. (a) In the Nu-B model, Ki-67-positive cells (brown) in tumors were detected by immunohistochemistry. Five fixed fields from each tumor section were analyzed for the number of Ki-67-positive cells. The percentage of Ki-67-positive cells was calculated by the number (Ki-67-positive cells/the total cell number of five fields) × 100%. The quantification of the percentage of Ki-67-positive cells is shown in the lower panel. (b) The protein expression levels ofp53 and p21 in tumors of Nu-B model were detected by Western blot. The quantification data are shown in the left panel. *indicates p < 0.05 versus the vehicle group.