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. 2015 Jan 21;10(1):e0115042. doi: 10.1371/journal.pone.0115042

Figure 8. GrEXPB2 and GrUBCEP12 proteins suppress virus resistance mediated by the N gene.

Figure 8

N. benthamiana leaves were co-infiltrated with Agrobacterium carrying binary vectors expressing PVX-GFP, N and P50 together with pEAQ35S expressing (a) 1, GrUBCEP12; 2, empty vector; (b) 1, GrEXPB2; 2, empty vector; 3, P0; 4, P38. GFP expression was visualized under UV illumination at 4 DPI. (c-d) Anti GFP immune blotting was performed on total protein samples taken at 4 DPI from N. benthamiana leaf patches co-expressing the combinations of constructs described in a and b. The number on the blot corresponds to the number on the leaf above each blot. Ponceau S staining (lower panel) was used to show equal loading.