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. 2014 Nov 24;15(1):1016. doi: 10.1186/1471-2164-15-1016

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© Tokunaga et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Experimental design. (A) Biosynthetic pathway of GPI-anchored proteins. GPI-anchor precursors are synthesized and attached to proteins in the endoplasmic reticulum (ER). The resulting GPI-anchored proteins are expressed at the cell surface. Out of 26 genes involved in this pathway, four (shown in red) were excluded from our screening (see text). Dol-P-Man, dolichol-phosphate-mannose. (B) Schematic screening procedure. (C) Functional complementation assay. Upper, principle of the assay. Lower, representative culture fields of Pigl ^m mutant ESCs unrestored (left) and restored (right) by plasmid transfection (scale bars, 50 μm). For clarity, original images shown in Additional file 1: Figure S1C were trimmed.