Figure 5. Turnover of PIP₃ by K13A PTEN in solutions of various salt concentrations.

Reactions were performed in 50 mM Tris–HCl buffer, pH 7.6, containing NaCl (0, 100, or 200 mM), EDTA (2.0 mM), MESG (0.20 mM), and DTBA (40 mM), and were initiated with the addition of K13A PTEN to 20 nM. Values are for maximum reaction velocity (± SE) at <10% turnover of substrate in reactions performed in triplicate or more. The resulting kinetic parameters are listed in Table 1.