Table 1. Kinetic parameters for catalysis by wild-type PTEN, K13A PTEN, and PTEN-L^a.

Enzyme	[NaCl] (mM)	kcat/KM (μM–1min–1)b	kcat (min–1)	KM (μM)	hc
Wild-type PTEN	0	174 ± 40	4000 ± 300	23 ± 5	1.00 ± 0.14
	100	108 ± 14c	2720 ± 130c	25 ± 3c	1.54 ± 0.16
	200	20 ± 4c	1430 ± 200c	70 ± 11c	2.93 ± 0.92
K13A PTEN	0	1.4 ± 0.4	91 ± 9	64 ± 16	1.25 ± 0.33
	100	1.0 ± 0.3	101 ± 12	100 ± 25	1.36 ± 0.35
	200	0.39 ± 0.13	139 ± 24	359 ± 96	1.35 ± 0.21
PTEN-L	0	100 ± 25	477 ± 28	4.8 ± 1.1	0.84 ± 0.30
	100	42 ± 8	515 ± 30	12 ± 2	1.23 ± 0.31
	200	10 ± 2	477 ± 40	47 ± 8	1.19 ± 0.17

^a Reactions were performed in 50 mM Tris–HCl buffer, pH 7.6, containing NaCl (0, 100, or 200 mM), EDTA (2.0 mM), MESG (0.20 mM), and DTBA (40 mM), and were initiated with the addition of PTEN to 20 nM.

^b Values (± SE) were derived by fitting initial velocity data to eq 1, unless noted otherwise.

^c Values (± SE) were derived by fitting initial velocity data to eq 2, and include k _cat/K _0.5 and K _0.5 rather than k _cat/K _M and K _M.