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. 2014 Dec 23;7:607. doi: 10.1186/s13071-014-0607-2

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© Lee et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PCR-RLB hybridization results for 23S-5S IGS fragments amplified from genomic DNA extracted from Rickettsia controls and A . americanum ticks. Next to the identification code of tick samples, Rickettsia species identifications as determined by the RLB hybridization patterns are shown in parentheses. Ra = R. amblyommii and Rm = R. montanensis. An example of hybridization patterns for some biotin-labeled A. americanum 23S-5S DNAs are presented in the lower half the figure. All of the ticks exhibited strong hybridization to the Rickettsia genus level (GP-RICK) and SFG (GP-SFG) probes, indicating that they were infected with a spotted fever group Rickettsia species. All but one 23S-5S DNA samples hybridized with probe P-AMB, indicating that these ticks were infected with R. amblyommii. Tick DNA sample 2FT365 hybridized with probe P-MAS/MON, but failed to hybridize to P-MAS/RHIPI, indicating that the tick was infected with R. montanensis.