TABLE 1A.
List of differentially expressed proteins identified by expression profiling in AT5BIVA and ATCL8 cells, respectively. Differentially expressed proteins belonging to functionally different classes are listed. Two-dimensional electrophoresis was performed for AT5BIVA and ATCL8 whole cell lysates with and without radiation exposure. The gel spots from radiation treated samples were compared to those without radiation treatment using Dymension (Syngene) image analysis software. The spots exhibiting differential normalized intensities were analyzed by MALDI-TOF mass spectrometry and identified using MASCOT database search. The listed proteins had confidence intervals greater than 95% and have at least 3 unique peptides associated with the protein identification. A green arrow depicts a down regulation subsequent to radiation treatment while a red arrow depicts an up-regulation.
| AT5BIVA | |||||
|---|---|---|---|---|---|
|
| |||||
| Protein Name | Accession Number | 30 min | 1h | 3h | 24h |
| Cytoskelton protein | |||||
| Vimentin | VIME_HUMAN | - | - |
|
|
| Ezrin | EZRI_HUMAN | - |
|
- | - |
| Actin, cytoplasmic 1 | ACTB_HUMAN | - | - |
|
|
| Tubulin beta–4 chain | TBB4_HUMAN |
|
- |
|
|
| Myosin regulatory light chain 2 | MLRM_HUMAN |
|
- |
|
|
| Cofilin, non-muscle isoform | COF1_HUMAN |
|
- | - | - |
| Transgelin | TAGL_HUMAN | - | - | - |
|
| Annexin A3 | ANXA3_HUMAN | - | - |
|
- |
| Vimentin | VIME_HUMAN | - | - |
|
- |
| Moesin | MOES_HUMAN | - |
|
|
- |
|
| |||||
| Signaling | |||||
|
| |||||
| Adenosine kinase | ADK_HUMAN | - | - |
|
- |
| Adenylate kinase | KAD2_HUMAN |
|
- | - |
|
| cAMP-dependent protein kinase | KAP3_HUMAN | - | - |
|
|
| ALK tyrosine kinase receptor precursor | ALK_HUMAN | - |
|
- | - |
| neutral alpha=glucosidase AB precursor | GANAB_HUMAN | - |
|
|
|
|
| |||||
| Transcription/Traslation/DNA Repair | |||||
|
| |||||
| Structural maintenance of chromosome | SMC1A_HUMAN | - |
|
- | - |
| Elongation factor 2 | EF2_HUMAN | - |
|
- | - |
| Nucleolin | NUCL_HUMAN | - |
|
- | - |
| Cellular nucleic acid binding protein | CNBP_HUMAN | - | - |
|
- |
| ATP dependent DNA helicase II | KU86_HUMAN | - | - |
|
- |
| Splicing factor | SFRS3_HUMAN | - | - |
|
|
| Exostosin-2 | EXT2_HUMAN | - |
|
- | - |
| Zinc finger protein 95 homolog | ZFP95_HUMAN | - |
|
- | - |
|
| |||||
| Cell Cycle/Apoptosis | |||||
|
| |||||
| Cell division protein kinase 6 | CDK6_HUMAN | - | - | - |
|
| 26S protease regulatory subunit 8 | PRS10_HUMAN | - | - |
|
|
| Ubiquitin-like 1 activating enzyme E1A | ULE1A_HUMAN | - | - |
|
- |
| Proteasome subunit alpah type 6 | PSA6_HUMAN |
|
- | - | - |
| Thioredoxin-like protein p19 precursor | TXNL2_HUMAN |
|
- |
|
|
| Cyclin B1 interacting protein 1 | CIP1_HUMAN | - |
|
- |
|
| 14-3-3 protein tau | 1433T_HUMAN | - | - |
|
|
| Heat shock protein HSP 90-alpha/beta | HS90A_HUMAN | - | - |
|
- |
| UV excision repair protein RAD23 homolog | RAD23B_HUMAN |
|
- | - | - |
| Microtubule-actin crosslinking factor 1 | MACF4_HUMAN |
|
- | - | - |
| Glyceraldehyde-3-phosphate dehydrogena | G3P2_HUMAN | - |
|
- | - |
| Superoxide dismutase[Cu-Zn] | SODC_HUMAN | - | - | - |
|