TABLE 1B.
List of differentially expressed proteins identified by expression profiling in AT5BIVA and ATCL8 cells, respectively. Differentially expressed proteins belonging to functionally different classes are listed. Two-dimensional electrophoresis was performed for AT5BIVA and ATCL8 whole cell lysates with and without radiation exposure. The gel spots from radiation treated samples were compared to those without radiation treatment using Dymension (Syngene) image analysis software. The spots exhibiting differential normalized intensities were analyzed by MALDI-TOF mass spectrometry and identified using MASCOT database search. The listed proteins had confidence intervals greater than 95% and have at least 3 unique peptides associated with the protein identification. A green arrow depicts a down regulation subsequent to radiation treatment while a red arrow depicts an up-regulation.
| ATGL8 | |||||
|---|---|---|---|---|---|
|
| |||||
| Protein Name | Accession Number | 30 min | 1h | 3h | 24h |
| Cytoskelton protein | |||||
| Annexin A1 | ANXA1_HUMAN |
|
|
- | - |
| Caldesmon | CALD_HUMAN |
|
- | - |
|
| Rab GDP dissiciation inhibitor alpha | GDIA_HUMAN |
|
- | - | - |
| Supervillin | SVIL_HUMAN | - |
|
- | - |
| Annexin A6 | ANXA6_HUMAN | - |
|
|
|
| Myosin heavy chain, skeletal muscle, fetal | MYH4_HUMAN | - | - |
|
|
| Transgelin-2 | TAGL2_HUMAN | - |
|
- | - |
| WD-repeat protein 22 | WDR22_HUMAN | - | - |
|
|
| Ezrin | EZRI_HUMAN |
|
- | - | |
| Myosin light chain kinase 2, skeletal/cardiac muscle | MYLK2_HUMAN |
|
|
|
|
|
| |||||
| Signaling | - | ||||
|
| |||||
| Protocadherin 15 precursor | PCD15_HUMAN |
|
|
|
|
| Mitigen-activated protein kinase kinase kinase kinase | M4K2_HUMAN | - |
|
- | - |
| Camp-specific 3′,5′-cyclic phosphodiesterase | PDE4B_HUMAN |
|
|
- | - |
| tumor necrosis factor | TNAP1_HUMAN |
|
- |
|
- |
| Phosphatidylinositol-glycan-specific phospholipase | PHL2_HUMAN |
|
- |
|
- |
| RasGAP-activating-like -protein 1 | RASL1_HUMAN |
|
|
|
|
| Protein tyrosine kinase 2 beta | FAK2_HUMAN | - | - |
|
- |
| Signal transducer and activator of transcription 3 | STAT3_HUMAN | - | - |
|
- |
| Guanine nucleotide-binding protein | GNAS_HUMAN | - | - |
|
- |
| Ephrin-B3 precursor | EFNB3_HUMAN | - | - |
|
- |
| Serine/threonine-protein kinase PAK 7 | PAK7_HUMAN | - | - |
|
- |
| Serine/threonine-protein kinase PAK 7 | PAK7_HUMAN |
|
|
||
| 130 kDa leucine-rich protein | LPPRC_HUMAN |
|
- | - | - |
| Vinculin | VINC_HUMAN |
|
- | - | - |
| Glyceraldehyde-3-phosphate dehydrogenase | G3P2_HUMAN | - | - |
|
- |
| ATP-dependent DNA helicase II, 70 kDa subunit | KU70_HUMAN |
|
- | - | - |
| Dedicator of cytokinesis protein 9 | DOCK9_HUMAN |
|
- |
|
- |
| Heat shock cognate 71 kDa protein | HSP7C_HUMAN | - | - |
|
|
|
| |||||
| Transcription/Translation/DNA Repair | |||||
|
| |||||
| Structural maintenance of chromosome 1 | SMCIA_HUMAN | - | - |
|
- |
| Transformation/transcription domain-associated protein | TRRAP_HUMAN | - |
|
|
|
| Chromodomain-helicase-DNA-binding protein 1 | CHD1_HUMAN | - |
|
|
|
| DNA-direct RNA polymerase L largest subunit | RPA1_HUMAN | - |
|
|
|
| RNA 3;-terminal phosphate cyclase-loke protein | RCL1_HUMAN |
|
- |
|
|
| Nucleolar protein | NOP5_HUMAN |
|
- |
|
- |
| Elongation factor 1-gamma | EF1G_HUMAN | - | - |
|
- |
| DNA-lyase | APEX1_HUMAN | - |
|
- | |
| Guanine nucleotide-binding prolein G, alpha subunit | GNA11_HUMAN | - |
|
|
|
| CENP-F kinetochore protein | CENPF_HUMAN | - |
|
|
|
| Nuclear receptor corepressor 1 | NCOR1_HUMAN | - | - |
|
- |
| Nucleosome assembly protein 1-like 4 | NP1L4_HUMAN | - | - |
|
|
|
| |||||
| Cell Cycle/Apoptosis | |||||
|
| |||||
| Proteosome subunit beta type1 | PSB1_HUMAN |
|
- | - | - |
| Ubiquitin ligase protein COP1 | COP1H_HUMAN | - |
|
|
- |
| 26S proteasome-associated protein | S1769_HUMAN | - | - |
|
|
| Ubiquitin-activating enzyme E1 | UBE1_HUMAN | - | - |
|
- |
| Proteasome subunit alpha type 7 | PSA7_HUMAN | - |
|
- | - |
| Programmed cell death 6-interacting protein | PDC6I_HUMAN | - |
|
- | - |
| G2 and S phase expressed protein 1 | GTSE1_HUMAN |
|
- | - | - |
| Angiopoietin-2 precursor | ANGP2_HUMAN | - | - |
|
|
| Stress-70 protein | GRP75_HUMAN | - |
|
|
|
| Nuclear autoantigenic sperm protein | NASP_HUMAN | - |
|
|
|