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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Bioorg Med Chem. 2014 Dec 8;23(3):602–611. doi: 10.1016/j.bmc.2014.11.043

Figure 5. Effects of IND-2 on mitochondrial membrane potential and DNA fragmentation.

Figure 5

(A). HCT-116 cells in complete medium were exposed to IND-2 (0, 1, or 10 [.proportional]M) for 4 hr. Cells were then treated with the reagents of the MitoTracker Red and Alexa Fluor 488 annexin V kits for flow cytometry. Representative results from two independent experiments, each performed in triplicate, are shown. (B) IND-2 induced DNA damage in HCT-116 cells. Chromosomal DNA was extracted from HCT-116 cells and resolved on a 1% agarose gel at 40 V for 6 hr. Lane 1: ‘M’, Marker; Lanes 2–3: untreated HCT cells, 500 ng and 1 μg, respectively; Lanes 4–5: HCT cells incubated with IND-2 (2.5 μM) for 6 hr.