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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Mol Cell Cardiol. 2014 Nov 18;79:295–302. doi: 10.1016/j.yjmcc.2014.11.013

Figure 2. Production of superoxide (O2·−) with rapid stimulation.

Figure 2

After 6hr of rapid stimulation in culture, O2·− production by atrial HL-1 cells was detected by dihydroethidium (DHE; 10μM; B, indicated by the bright red color) that is not present in control, spontaneously-beating cells (A). Superoxide generation was essentially abolished under these conditions by co-incubation of cells with the cell-permeable superoxide dismutase mimetic tempol (C). Scale bars = 10 microns.