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. 2014 Dec 5;15(1):1073. doi: 10.1186/1471-2164-15-1073

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© Vijaya Satya and DiCarlo; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Enzymatic primer digestion procedure. The ‘U’s indicate Uracil bases in the primers. These bases are placed as close the 3’ end of the primer as possible to maximize the number of primer bases removed.